|Name:||James Alwine, Ph.D.|
|Title/Titles:||Professor, Cancer Biology
Assoc Director for Core Facilities, Abramson Cancer Center
Investigator, Abramson Family Cancer Research Institute
ALWINE LAB OVERVIEWDr. Alwine studies how DNA viral infections deal with the consequences of inducing cellular stress responses. During infection by DNA viruses, such as human cytomegalovirus (HCMV; a herpesvirus) and simian virus 40 (SV40), cellular stress responses are triggered due to the stress of the viral infection; for example, the stress resulting from the greatly increased metabolic and synthetic rates needed for successful viral replication. more
Dr. Alwine is Professor of Cancer Biology and an Investigator in the Abramson Family Cancer Research Institute at the University of Pennsylvania .He received his B.S. in Chemistry from Elizabethtown College in 1969 and his Ph.D. in Biological Chemistry from Pennsylvania State University in 1974. His thesis work involved the analysis of herpes simplex virus genome structure and transcription. He then moved to the laboratory of George Stark in the Biochemistry Department of Stanford University where he began what would become a career-long fascination with Simian virus 40 (SV40). During this period he also developed a technique for blotting and analyzing RNA, which he named the Northern. In 1977 he joined George Khoury's laboratory at NIH as a staff fellow and continued his work on SV40. In 1980 he accepted an assistant professor position at the University of Pennsylvania School of Medicine and has not moved since. He has continued his work with SV40 and has also returned to his studies of herpes viruses utilizing the human cytomegalovirus. At Penn he has served as Interim Chair of Microbiology; Program Director for the Tumor Virology Program of the Abramson Cancer Center; Director of the Microbiology and Virology Program of the Cell and Molecular Biology Graduate Group and Chairman of the Cell and Molecular Biology Graduate Group. Presently he serves as Associate Director of the Abramson Cancer Center, Assistant Dean in the School of Medicine and Director of the Cell Growth and Cancer Graduate Program. He has been the mentor for 16 Ph.D. students and 10 postdoctoral researchers. In 1997, he was honored by receiving the Dean's Award for Excellence in Graduate Student Teaching. Outside of Penn, Dr. Alwine served as Editor of Molecular and Cellular Biology for 10 years and on the editorial boards of several journals including the Journal of Virology. He has been a member of several NIH study sections and review groups. In 1994 he was elected a Fellow of the American Academy of Microbiology.
Selected PublicationsHans H., Alwine JC.: Functionally significant secondary structure of the simian virus 40 late polyadenylation signal. Molecular & Cellular Biology 20(8): 2926-32, Apr 2000.
Cooke C., Hans H., Alwine JC.: Utilization of splicing elements and polyadenylation signal elements in the coupling of polyadenylation and last-intron removal. Molecular & Cellular Biology 19(7): 4971-9, Jul 1999.
Lukac DM., Alwine JC.: Effects of human cytomegalovirus major immediate-early proteins in controlling the cell cycle and inhibiting apoptosis: studies with ts13 cells. Journal of Virology 73(4): 2825-31, Apr 1999.
Lutz CS., Cooke C., O'Connor JP., Kobayashi R., Alwine JC.: The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. Rna 4(12): 1493-9, Dec 1998.
Harel NY., Alwine JC.: Phosphorylation of the human cytomegalovirus 86-kilodalton immediate-early protein IE2. Journal of Virology 72(7): 5481-92, Jul 1998.
Damania B., Lieberman P., Alwine JC.: Simian virus 40 large T antigen stabilizes the TATA-binding protein-TFIIA complex on the TATA element. Molecular & Cellular Biology 18(7): 3926-35, Jul 1998.
Damania B., Mital R., Alwine JC.: Simian virus 40 large T antigen interacts with human TFIIB-related factor and small nuclear RNA-activating protein complex for transcriptional activation of TATA-containing polymerase III promoters. Molecular & Cellular Biology 18(3): 1331-8, Mar 1998.
O'Connor JP., Alwine JC., Lutz CS.: Identification of a novel, non-snRNP protein complex containing U1A protein. Rna 3(12): 1444-55, Dec 1997.